EXOSC8 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXOSC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (2)

Cat no : 11979-1-AP

Synonyms

RRP43, Ribosomal RNA-processing protein 43, p9, Opa-interacting protein 2, OIP2

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Validation Data Gallery

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  WB analysis of HEK-293 using 11979-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11979-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11979-1-AP

  IP result of anti-EXOSC8 (IP:11979-1-AP, 4ug; Detection:11979-1-AP 1:500) with HEK-293 cells lysate 1560ug.

• 

  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of 4T1 using 11979-1-AP

  Immunofluorescent analysis of 4T1 cells, using EXOSC8 antibody 11979-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC8 fusion protein Ag2605
• Full Name: exosome component 8
• Calculated Molecular Weight: 276 aa, 30 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC020773
• Gene Symbol: EXOSC8
• Gene ID (NCBI): 11340
• RRID: AB_2101981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.

Publications

Species	Application	Title
human	WB	Nat Commun; Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.; Authors - Caitlin A Nichols ; KO Validated
human	WB	Nat Commun; EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.; Authors - Veronika Boczonadi ; KD Validated
human	WB	PLoS One; Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA.; Authors - Saeki Makio M
human	WB	Neuromuscul Disord; New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient.; Authors - María Elena Rodríguez-García
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were used. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC8 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11979-1-AP)

FH

Nikolaus (Verified Customer) (05-24-2022)

Antibodies applied after 2 rounds of stripping, signal still satisfactory

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: mESC

EXOSC8 Antibody Western Blot validation (1/2000 dilution) in mESC (Cat no:11979-1-AP)