HJURP Polyclonal antibody

• Featured Product
• KD/KO Validated

HJURP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15283-1-AP

Synonyms

FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9

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Validation Data Gallery

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  WB analysis using 15283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15283-1-AP (HJURP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, A549 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Product Information

15283-1-AP targets HJURP in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HJURP fusion protein Ag7402
• Full Name: Holliday junction recognition protein
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC001940
• Gene Symbol: HJURP
• Gene ID (NCBI): 55355
• RRID: AB_2116879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

Publications

Species	Application	Title
human	WB	Dev Cell; CENP-A Ubiquitylation Is Indispensable to Cell Viability.; Authors - Yohei Niikura
human	IHC	Front Cell Dev Biol; Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.; Authors - Weihao Lin
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	J Biol Chem; Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.; Authors - Jianyu Wang ; KD Validated
human	WB	Biochem Biophys Res Commun; Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.; Authors - Ye Li ; KD Validated
		J Vis Exp; Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).; Authors - Yohei Niikura