CPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 18703-1-AP

Synonyms

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial

---

Validation Data Gallery

• 

  WB analysis of HeLa using 18703-1-AP

  WB result of CPS1 antibody (18703-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPS1 transfected HeLa cells.

• 

  WB analysis using 18703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18703-1-AP (CPS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18703-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18703-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18703-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18703-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (18703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 18703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (18703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18703-1-AP targets CPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: carbamoyl-phosphate synthetase 1, mitochondrial
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 140-165 kDa
• GenBank Accession Number: BC140943
• Gene Symbol: CPS1
• Gene ID (NCBI): 1373
• RRID: AB_2084238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala
mouse	IF	Nat Commun; Plasmodium sporozoite phospholipid scramblase interacts with mammalian carbamoyl-phosphate synthetase 1 to infect hepatocytes.; Authors - Sung-Jae Cha
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
mouse	WB	iScience; Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions; Authors - Xinghong Guo
human	WB	J Cell Mol Med; Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells.; Authors - Xi Liu ; KD Validated
human	WB	Front Cell Dev Biol; Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.; Authors - Changsen Bai ; KD Validated