CENPH Polyclonal antibody

CENPH Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12841-1-AP

Synonyms

CENP H, CENPH, centromere protein H, ICEN35, NNF1, PMF1

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Validation Data Gallery

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  WB analysis using 12841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12841-1-AP (CENPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12841-1-AP

  IP result of anti-CENPH (IP:12841-1-AP, 4ug; Detection:12841-1-AP 1:1000) with HeLa cells lysate 1600 ug.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12841-1-AP targets CENPH in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPH fusion protein Ag3836
• Full Name: centromere protein H
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC015355
• Gene Symbol: CENPH
• Gene ID (NCBI): 64946
• RRID: AB_2076634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centromere protein H (CENPH) is a component of the active centromere complex that associates with centromeric DNA and binds spindle microtubules to the chromosomes, which is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPH plays a key role in assembly of kinetochore proteins, mitotic progression and chromosome segregation that may cause misaligned chromosomes and multipolar spindles. Besides, CENPH may be associated with the modulation of the cell cycle through an interaction with CENPC. It has been shown that the expression of CENPH is related to clinical application including colorectal cancer, gastric cancer, renal cell carcinoma, hepatocellular carcinoma and other poor prognosis in various solid tumors. (PMID: 26378239, 27993978, 28819418)

Publications

Species	Application	Title
mouse	WB	Neuro Oncol; Mitogen-Induced Defective Mitosis Transforms Neural Progenitor Cells; Authors - Hiba K Omairi
human	WB	Am J Cancer Res; CENPH overexpression promotes the progression, cisplatin resistance, and poor prognosis of lung adenocarcinoma via the AKT and ERK/P38 pathways; Authors - Qiao Li ; KD Validated