Nestin Monoklonaler Antikörper

Nestin Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

32

CloneNo.

1D11H2

Kat-Nr. : 66259-1-Ig

Synonyme

NES, Nestin

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Galerie der Validierungsdaten

• 

  WB analysis of Neuro-2a using 66259-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66259-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66259-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66259-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293-Zellen, PC-12-Zellen, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• FC: See 2 publications below

Produktinformation

66259-1-Ig bindet in WB, FC, ELISA Nestin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nestin
• Berechnetes Molekulargewicht: 177 kDa
• Beobachtetes Molekulargewicht: 150-170 kDa
• GenBank-Zugangsnummer: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human		Cell Res; RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability.; Authors - Feng Xiong
mouse	FC	J Allergy Clin Immunol; Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1.; Authors - Xia Ke
rat		Small; Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.; Authors - Xiheng Lu
mouse	WB	EBioMedicine; Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.; Authors - Guangtian Wang
rat		Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi

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