NR1H3 Polyklonaler Antikörper

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• KD/KO Validated

NR1H3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

79

Rezensionen (1)

Kat-Nr. : 14351-1-AP

Synonyme

Liver X receptor alpha, LXR a, LXRA, LXRα, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Galerie der Validierungsdaten

• 

  WB analysis using 14351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14351-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, COLO 320-Zellen, Mauslebergewebe, Rattenlebergewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 78 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

14351-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA NR1H3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR1H3 fusion protein Ag5697
• Vollständiger Name: nuclear receptor subfamily 1, group H, member 3
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC041172
• Gene symbol: NR1H3
• Gene ID (NCBI): 10062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.

Publikationen

Species	Application	Title
	WB	Nat Commun; p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1; Authors - Min Yan Shi
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	WB,IF	Food Res Int; Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota; Authors - Xinlong Cheng
mouse	WB	Br J Pharmacol; Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis.; Authors - Chuanrui Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (06-10-2021)

works good in rat and human cells form western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: rat and human liver cells

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