NCAM1/CD56 Monoklonaler Antikörper

NCAM1/CD56 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E8C9

Kat-Nr. : 60238-1-Ig

Synonyme

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1, NCAM1/CD56



Geprüfte Anwendungen

Erfolgreiche Detektion in WBfetales humanes Hirngewebe, C6-Zellen, Hausschwein-Hirngewebe, humanes Hirngewebe
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, humanes Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunhistochemie (IHC)IHC : 1:2000-1:8000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60238-1-Ig bindet in WB, IHC, IF, ELISA NCAM1/CD56 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen NCAM1/CD56 fusion protein Ag5732
Vollständiger Name neural cell adhesion molecule 1
Berechnetes Molekulargewicht 95 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protokolle

Produktspezifische Protokolle
WB protocol for NCAM1/CD56 antibody 60238-1-IgProtokoll herunterladen
IHC protocol for NCAM1/CD56 antibody 60238-1-IgProtokoll herunterladen
IF protocol for NCAM1/CD56 antibody 60238-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Small

Nanobody-Engineered Natural Killer Cell Conjugates for Solid Tumor Adoptive Immunotherapy

Authors - Liang Gong
humanIF

J Transl Med

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease

Authors - Beibei Li
humanWB

Stem Cells Dev

Effect of the Soluble Factors Released by Dental Apical Papilla-Derived Stem Cells on the Osteo/Odontogenic, Angiogenic, and Neurogenic Differentiation of Dental Pulp Cells.

Authors - Shi Yu
humanIHC

Jpn J Clin Oncol

Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter.

Authors - Yan Huang
human,mouseIF

Hepatology

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis

Authors - Xianzhi Liu