NKG2A Polyclonal antibody

NKG2A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10935-1-AP

Synonyms

KLRC1, NKG2-A/B-activating NK receptor, NKG2, NK cell receptor A, CD159A

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Validation Data Gallery

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  WB analysis of human liver using 10935-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10935-1-AP (NKG2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10935-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10935-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 10935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NKG2A antibody (10935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10935-1-AP targets NKG2A in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NKG2A fusion protein Ag1376
• Full Name: killer cell lectin-like receptor subfamily C, member 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC012550
• Gene Symbol: NKG2A
• Gene ID (NCBI): 3821
• RRID: AB_2249683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLRC1, also known as NKG2A (NKG2) and CD159A, is a single-pass type II membrane protein with extracellular C-type lectin domain. It belongs to the natural killer cell lectin-like receptor family, which is a group of transmembrane proteins preferentially expressed in NK cells. KLRC1 can form a heterodimer with CD94. The NKG2A/CD94 complex is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells.

Publications

Species	Application	Title
		PLoS One; Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis.; Authors - Yi Li
		Med; Targeting HLA-E-overexpressing cancers with a NKG2A/C switch receptor; Authors - Michelle Sætersmoen