Validation Data Gallery
|Positive WB detected in||mouse brain tissue, mouse cerebellum tissue, rat brain tissue, pig brain tissue|
|Positive IHC detected in||human lung cancer tissue, human appendicitis tissue, human colon tissue, human gliomas tissue, human tonsillitis tissue, Insulinoma tissue, mouse brain tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:20000|
|Sample-dependent, check data in validation data gallery|
14255-1-AP targets NCAM1/CD56 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NCAM1/CD56 fusion protein Ag5528|
|Full Name||neural cell adhesion molecule 1|
|Calculated molecular weight||95 kDa|
|Observed molecular weight||120 kDa, 140 kDa, 180 kDa|
|GenBank accession number||BC047244|
|Gene ID (NCBI)||4684|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes.
Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activity.
PolySia-NCAM modulates the formation of ductular reactions in liver injury.
J Clin Invest
Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells.
Clin Cancer Res
Quantitative Map of Proteome Dynamics during Neuronal Differentiation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kenzo (Verified Customer) (01-09-2023)
Worked great. The staining pattern was consistent with what has been reported.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue @ 1:1000. We used a Tris-EDTA antigen retrieval.
Toni (Verified Customer) (02-28-2019)
IP conditions:1ug of rbt anti-NCAM1 was added to 100ug human cortex homogenate (0.5ug/ul) in Tris-sucrose buffer with 1% SDS and 1% Triton X100 thenincubated rotating ON at 4C. Sample was then added to 50ul TBST-washed sheep anti-rabbit magnetic beads and incubated rotating 3hr at 4C. Following 4 TBST washes, bound proteins were eluted with 30ul of elution buffer containing SDS and BME. WB conditions:Samples subjected to SDS-PAGE on 4-12% Bis-Tris gels followed by semi-dry transfer to nitrocellulose membranes using standard conditions. Membranes were blocked for 1hr at RT in 50% LiCor Odyssey blocking buffer (TBS) then probed with either 1:5000 (v/v) rabbit anti-NCAM1 (Proteintech) or 1:1000 (v/v) goat anti-NCAM1 (R&D Systems) in 50% LiCor Odyssey blocking buffer (TBS + 0.05% Tween-20) ON at 4C. Following TBS+ 0.1% Tween-20 washes, membranes were incubated with the appropriate IR-dye labeled secondary antibody for 1hr at RT, TBST washed, then scanned.*you have my permission to edit the comments above and crop, but not alter, the image provided if you wish to remove reference to an antibody from another company.