Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (4)

Cat no : 10529-1-AP

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A

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Validation Data Gallery

• 

  WB analysis of PC-12 using 10529-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10529-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10529-1-AP (Chromogranin A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10529-1-AP (Chromogranin A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10529-1-AP (Chromogranin A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10529-1-AP (Chromogranin A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10529-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10529-1-AP (Chromogranin A antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse pancreas using 10529-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 10529-1-AP (Chromogranin A antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 10529-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (10529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue, mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

10529-1-AP targets Chromogranin A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Chromogranin A fusion protein Ag0807
• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: Chromogranin A
• Gene ID (NCBI): 1113
• RRID: AB_2081122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publications

Species	Application	Title
mouse	IF	Cell Metab; Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance.; Authors - Yanru Wu
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang
human	WB	Sci Rep; Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line.; Authors - Azita Monazzam
human	WB	Discov Oncol; Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma; Authors - Hao Liu
mouse	IHC	Dose Response; The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.; Authors - Danjie Liu
human	WB	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

balawant (Verified Customer) (07-26-2022)

This Antibody is working great. I have used for colon and colon cancer cell line

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Susmita (Verified Customer) (04-13-2022)

This antibody works excellent.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500
• Cell Tissue Type: PC cell line

FH

James (Verified Customer) (03-31-2021)

Antibody worked well in both recombinant Chromogranin A produced in both HeLa cell lysates and in HEK cells overexpressed with Chromogranin A

• Applications: Western Blot, Cell culture
• Cell Tissue Type: HeLa cell lysate IVT expression and HEK cell overexpression

FH

Iram (Verified Customer) (08-14-2020)

Used 1:1000 dilution for immunoblotting. It gives a very clean band.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer

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