MMP7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MMP7 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

87

Kat-Nr. : 10374-2-AP

Synonyme

MMP 7, Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin

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Galerie der Validierungsdaten

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  WB analysis using 10374-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10374-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10374-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10374-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 10374-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MMP7 antibody (10374-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, humanes Plazenta-Gewebe, NIH/3T3-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Pankreaskarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 72 publications below
• IHC: See 18 publications below
• IF: See 1 publications below

Produktinformation

10374-2-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA MMP7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMP7 fusion protein Ag0550
• Vollständiger Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC003635
• Gene symbol: MMP7
• Gene ID (NCBI): 4316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Publikationen

Species	Application	Title
human	WB	Cancer Res; Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis.; Authors - Xiaofeng Wang
human	WB	Cancer Res; Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation.; Authors - Rongfa Yuan
human	IHC	Cancer Res; KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.; Authors - Peng Yuan
human	WB	Oncogene; Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.; Authors - Q Zhou
human	WB	Clin Cancer Res; BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.; Authors - Zebing Liu
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan

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