MGEA5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MGEA5 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

62

Rezensionen (1)

Kat-Nr. : 14711-1-AP

Synonyme

Beta N acetylhexosaminidase, Bifunctional protein NCOAT, HAT, HEXC, Hexosaminidase C, KIAA0679, MEA5, Meningioma expressed antigen 5, MGEA5, N acetyl beta glucosaminidase, NCOAT, O GlcNAcase, OGA

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Galerie der Validierungsdaten

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  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14711-1-AP

  WB result of MGEA5 antibody (14711-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGEA5 transfected HepG2 cells.

• 

  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14711-1-AP

  IP result of anti-MGEA5(IP:14711-1-AP, 4ug; Detection:14711-1-AP 1:2000) with HepG2 cells lysate 1880 ug.

• 

  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 14711-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MGEA5 (14711-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SMMC-7721-Zellen, BxPC-3-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Meningiom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14711-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA MGEA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGEA5 fusion protein Ag6405
• Vollständiger Name: meningioma expressed antigen 5 (hyaluronidase)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC039583
• Gene symbol: MGEA5
• Gene ID (NCBI): 10724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
rat	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
mouse	WB	Proc Natl Acad Sci U S A; Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.; Authors - Myers Samuel A SA
Drosophila	WB	Proc Natl Acad Sci U S A; O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.; Authors - Pablo T Radermacher
human	WB	Leukemia; MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.; Authors - An-Ping Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (02-28-2019)

Always two bands together

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

MGEA5 Antibody Western Blot, validation (1:1000 dilution) in C2C12 (Cat no:14711-1-AP)

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