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TAU Polyklonaler Antikörper

TAU Polyklonal Antikörper für WB, IHC, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-Fro, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10274-1-AP

Synonyme

MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using TAU Polyclonal antibody (10274-1-AP)

    WB analysis of mouse brain using 10274-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using TAU Polyclonal antibody (10274-1-AP)

    IHC staining of human brain using 10274-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TAU Polyclonal antibody (10274-1-AP)

    IHC staining of human brain using 10274-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TAU Polyclonal antibody (10274-1-AP)

    IHC staining of human gliomas using 10274-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TAU Polyclonal antibody (10274-1-AP)

    IHC staining of human gliomas using 10274-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAU Polyclonal antibody (10274-1-AP)

    IF Staining of mouse brain using 10274-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAU Polyclonal antibody (10274-1-AP)

    IF Staining of mouse brain using 10274-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, rat brain
Erfolgreiche Detektion in IHChumanes Hirngewebe, humanes Gliomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-FroMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10274-1-AP bindet in WB, IHC, IF-Fro, ELISA TAU und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TAU fusion protein Ag0354
Vollständiger Name microtubule-associated protein tau
Berechnetes Molekulargewicht 37-46, 79-81 kDa
Beobachtetes Molekulargewicht 45-70 kDa, 100 kDa
GenBank-ZugangsnummerBC000558
Gene symbol TAU
Gene ID (NCBI) 4137
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

Protokolle

Produktspezifische Protokolle
WB protocol for TAU antibody 10274-1-APProtokoll herunterladen
IHC protocol for TAU antibody 10274-1-APProtokoll herunterladen
IF protocol for TAU antibody 10274-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang
View Article
human

Brain

Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.

Authors - Lina Pan
View Article
mouseIHC

Hum Mol Genet

Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice.

Authors - Rodríguez-Navarro Jose A JA
View Article
ratWB

Acta Neuropathol Commun

Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast.

Authors - Georgina Perez Garcia
View Article
mouse,ratWB

Free Radic Biol Med

The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro

Authors - Jie Ai
View Article
mouseIF

Mol Ther Nucleic Acids

Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.

Authors - Li Zeng
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

manohar (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neuronal stem cells
FH

David (Verified Customer) (01-02-2024)

Produces a single band at around 45kDa which is concordant with the data sheet.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y lysate
FH

Tanusree (Verified Customer) (02-14-2019)

The antibody worked well with the mouse primary cells and tissue.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse brain