MAP2 Polyklonaler Antikörper

MAP2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

217

Rezensionen (6)

Kat-Nr. : 17490-1-AP

Synonyme

MAP 2, MAP2, MAP2A, MAP2B, MAP2C

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Galerie der Validierungsdaten

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  WB analysis using 17490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17490-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17490-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17490-1-AP

  IP Result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

• 

  IP experiment of mouse brain using 17490-1-AP

  IP result of anti-MAP2(IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:8000) with mouse brain tissue lysate 1280 ug.

• 

  IP experiment of SH-SY5Y using 17490-1-AP

  IP result of anti-MAP2(IP:17490-1-AP, 4ug; Detection:17490-1-AP 1:10000) with SH-SY5Y cells lysate 1240 ug.

• 

  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17490-1-AP (MAP2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17490-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of iPS cells using 17490-1-AP

  Immunofluorescent staining of MAP2 (17490-1-AP, 1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

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  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

• 

  IF Staining of mouse brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 17490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 17490-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human MAP2 (17490-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

• 

  FC experiment of Neuro-2a using 17490-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (17490-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, iPS-Zellen, Maushirngewebe
• Erfolgreiche Detektion in FC: Neuro-2a-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 60 publications below
• IHC: See 27 publications below
• IF: See 150 publications below
• FC: See 1 publications below

Produktinformation

17490-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP2 fusion protein Ag11580
• Vollständiger Name: microtubule-associated protein 2
• Berechnetes Molekulargewicht: 200 kDa
• Beobachtetes Molekulargewicht: 280 kDa, 70-85 kDa
• GenBank-Zugangsnummer: BC038857
• Gene symbol: MAP2
• Gene ID (NCBI): 4133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.

What is the molecular weight of MAP2? Is MAP2 post-translationally modified?

MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).

What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?

MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.

Can MAP2 be used as a neuronal marker?

MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).

Publikationen

Species	Application	Title
mouse	IF	Cell Stem Cell; In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.; Authors - Wenjiao Tai ; KO Validated
human	IF	Cell Stem Cell; Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.; Authors - Elke Gabriel
mouse	WB,IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	WB	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
mouse	IHC,IF	Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
human	IF	Ann Neurol; Mutant huntingtin is present in neuronal grafts in Huntington's disease patients.; Authors - F Cicchetti

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Filipa (Verified Customer) (09-18-2023)

I used this anti-MAP2 antibody at 1:200 dilution for IF. The cells were fixed in 4% PFA in PBS for 20 minutes, and incubated with the primary antibody for 3 hours at RT. MAP2 staining works very well at this dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: primary rat hippocampal neurons

MAP2 Antibody Immunofluorescence validation (1:200 dilution) in primary rat hippocampal neurons (Cat no:17490-1-AP)

FH

BING (Verified Customer) (08-04-2021)

suitable for detection of both bands of MAP2 by WB with one above 250 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: primary cortical neuron lysate

MAP2 Antibody Western Blot validation (1:1000 dilution) in primary cortical neuron lysate (Cat no:17490-1-AP)

FH

Dipen (Verified Customer) (07-20-2020)

Not great for Western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Hippocampal neurons

MAP2 Antibody Western Blot, validation (1:500 dilution) in Hippocampal neurons (Cat no:17490-1-AP)

FH

Naomi (Verified Customer) (03-09-2020)

I used the rabbit polyclonal anti-MAP2 antibody at 1:500 dilution to in differentiated PC-12 cells. The cells were fixed in 4% PFA in PBS for 12 minutes. 1 hr in blocking buffer, followed by 24 h incubation in primary antibody. MAP2 was expressed in neurites. Works well at this dilution, great images.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: PC-12

FH

Gloria (Verified Customer) (01-30-2020)

Beautiful staining in primary hippocampal and cortical neurons.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 dilution
• Cell Tissue Type: Primary hippocampal and cortical neurons

MAP2 Antibody Immunofluorescence, validation (1:250 dilution dilution) in Primary hippocampal and cortical neurons (Cat no:17490-1-AP)

FH

Ricky (Verified Customer) (02-22-2019)

1:5000 dilution is good enough for a clear and sharp image of western blot. I am now using Li-Cor's Odyssey CLX imaging systems . The second antibody is from Li-Cor with 1:5000 dilution. By the way, I also tried to use 1:1000 dilution for my low expression samples, and there was no bad noise backgrounds.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

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