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MACF1 Polyklonaler Antikörper
MACF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13058-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | U-87 MG-Zellen, Mauslungengewebe, NIH/3T3-Zellen |
Erfolgreiche Detektion in IHC | humanes Lungengewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | U-251-Zellen, NIH/3T3-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 3 publications below |
Produktinformation
13058-1-AP bindet in WB, IHC, IF/ICC, ELISA MACF1 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | MACF1 fusion protein Ag3730 |
Vollständiger Name | microtubule-actin crosslinking factor 1 |
Berechnetes Molekulargewicht | 5430 aa, 620 kDa |
Beobachtetes Molekulargewicht | 600 kDa |
GenBank-Zugangsnummer | BC007330 |
Gene symbol | MACF1 |
Gene ID (NCBI) | 23499 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for MACF1 antibody 13058-1-AP | Protokoll herunterladen |
IHC protocol for MACF1 antibody 13058-1-AP | Protokoll herunterladen |
IF protocol for MACF1 antibody 13058-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Nat Commun The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers. | ||
Cell Death Differ MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.
| ||
PLoS Genet BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. | ||
Oncotarget LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics. | ||
Biomed Pharmacother Proteomics-based identification of tumor relevant proteins in lung adenocarcinoma. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Joleen (Verified Customer) (06-03-2019) | MACF1 should be the top band but there were a lot of non specific bands as well.
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