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  • KD/KO Validated

MACF1 Polyklonaler Antikörper

MACF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13058-1-AP

Synonyme

MACF, KIAA0754, Actin cross-linking family protein 7, ACF7, ABP620



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU-87 MG-Zellen, Mauslungengewebe, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Lungengewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU-251-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13058-1-AP bindet in WB, IHC, IF/ICC, ELISA MACF1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MACF1 fusion protein Ag3730
Vollständiger Name microtubule-actin crosslinking factor 1
Berechnetes Molekulargewicht 5430 aa, 620 kDa
Beobachtetes Molekulargewicht600 kDa
GenBank-ZugangsnummerBC007330
Gene symbol MACF1
Gene ID (NCBI) 23499
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.

Protokolle

Produktspezifische Protokolle
WB protocol for MACF1 antibody 13058-1-APProtokoll herunterladen
IHC protocol for MACF1 antibody 13058-1-APProtokoll herunterladen
IF protocol for MACF1 antibody 13058-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
humanWB

Nat Commun

The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.

Authors - Yaodong Zhang
mouseWB

Cell Death Differ

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Authors - Lifang Hu
  • KD Validated
mouseWB

PLoS Genet

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

Authors - Ying Hsu
humanWB

Oncotarget

LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics.

Authors - Qian Yao
humanIHC

Biomed Pharmacother

Proteomics-based identification of tumor relevant proteins in lung adenocarcinoma.

Authors - Xin Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Joleen (Verified Customer) (06-03-2019)

MACF1 should be the top band but there were a lot of non specific bands as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
MACF1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13058-1-AP)