JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 10023-1-AP

Synonyme

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 10023-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10023-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10023-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10023-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10023-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10023-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10023-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10023-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10023-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10023-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, RAW 264.7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Lungenkarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 28 publications below
• IHC: See 3 publications below

Produktinformation

10023-1-AP bindet in WB, IHC, IF/ICC, ELISA JNK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag0019
• Vollständiger Name: mitogen-activated protein kinase 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa, 50-55 kDa
• GenBank-Zugangsnummer: NM_138982
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publikationen

Species	Application	Title
mouse	WB	J Pineal Res; Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high fat diet-fed mice.; Authors - Jie Yin
human		Front Oncol; Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.; Authors - Dan-Dan Lin
human	WB	Neuropharmacology; TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation.; Authors - Ah-Reum Ko
rat	WB	Ecotoxicol Environ Saf; PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats.; Authors - Yang Yang
mouse	WB	J Biol Chem; Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation.; Authors - Nadiya Byts
human	WB	Front Pharmacol; BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model; Authors - Zhuo Lu

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