INVS Polyklonaler Antikörper

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• KD/KO Validated

INVS Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10585-1-AP

Synonyme

INV, inversin, INVS, KIAA0573, Nephrocystin 2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10585-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10585-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10585-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10585-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10585-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10585-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 10585-1-AP

  IF result from Dr. Corbit, Kevin. anti-INVS (also named as NPHP2; 10585-1-AP) marks the ‘inversin compartment’ and basal bodies of Human hTERT-RPE1 cells.

• 

  IF Staining of HeLa using 10585-1-AP

  Immunofluorescent analysis of Hela cells, using INVS antibody 10585-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen, Maus-Skelettmuskelgewebe, Raji-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

10585-1-AP bindet in WB, IF, FC, IHC, ELISA INVS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INVS fusion protein Ag0934
• Vollständiger Name: inversin
• Berechnetes Molekulargewicht: 118 kDa, 100 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC006370
• Gene symbol: INVS
• Gene ID (NCBI): 27130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.

Publikationen

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated
	IF	Proc Natl Acad Sci U S A; A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia.; Authors - Natalie Acker
rat	WB	Endocrinology; PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.; Authors - Linxi Li ; KD Validated
rat	IHC	Virchows Arch; Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats.; Authors - Leh Sabine S
human	IF	Am J Med Genet A; Early presentation of cystic kidneys in a family with a homozygous INVS mutation.; Authors - Machteld M Oud
human	FC	JCI Insight; Cilia proteins are biomarkers of altered flow in the vasculature.; Authors - Ankan Gupta

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