Mitofilin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Mitofilin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

86

Rezensionen (6)

Kat-Nr. : 10179-1-AP

Synonyme

IMMT, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

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Galerie der Validierungsdaten

• 

  WB analysis using 10179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10179-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10179-1-AP (Mitofilin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10179-1-AP

  IP result of anti-Mitofilin (IP:10179-1-AP, 3ug; Detection:10179-1-AP 1:1000) with Raji cells lysate 3800ug.

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10179-1-AP (Mitofilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10179-1-AP (Mitofilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10179-1-AP (Mitofilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10179-1-AP (Mitofilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10179-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mitofilin antibody (10179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HEK-293T using 10179-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Mitofilin (10179-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, MCF-7-Zellen, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 75 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

10179-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Mitofilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mitofilin fusion protein Ag0102
• Vollständiger Name: inner membrane protein, mitochondrial (mitofilin)
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC002412
• Gene symbol: IMMT
• Gene ID (NCBI): 10989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Publikationen

Species	Application	Title
human		Nat Methods; Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.; Authors - Mark Bates
human	WB	Cell Metab; Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism; Authors - Jee Hyung Sohn
mouse	WB	Cell Metab; A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.; Authors - Pedro Latorre-Muro
	WB	Cell Metab; The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.; Authors - Carmelina Petrungaro
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
human	WB	Mol Cell; The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation.; Authors - Katia Cosentino

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mia (Verified Customer) (09-28-2022)

very clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mitochondria protein

FH

siiting (Verified Customer) (03-20-2022)

very good antibody, second time buy already

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

FH

Maria (Verified Customer) (08-15-2021)

Mitofilin in human primary fibroblasts. Blocking with 5% milk in PBST (0.1%t tween) 1h RT. Primary ab 1:1000 in BSA 3% in PBST O/N at 4º incubation. Secondary 1:5000 HRP 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

Mitofilin Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:10179-1-AP)

FH

CYNTHIA (Verified Customer) (07-16-2020)

I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues

• Applications: Western Blot

FH

SITING (Verified Customer) (07-13-2020)

SHOW VERY STRONG SIGNAL

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Yan (Verified Customer) (01-28-2020)

This antibody worked very well on mouse heart tissue for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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