DISC1-long-specific Polyclonal antibody

DISC1-long-specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15500-1-AP

Synonyms

C1orf136, DISC1, DISC1 long, DISC1-long-specific, disrupted in schizophrenia 1, KIAA0457, SCZD9

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Validation Data Gallery

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  IHC staining of human brain using 15500-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15500-1-AP (DISC1-long-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15500-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15500-1-AP (DISC1-long-specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15500-1-AP targets DISC1-long-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: disrupted in schizophrenia 1
• Calculated Molecular Weight: 95 kDa
• GenBank Accession Number: NM_018662
• Gene Symbol: DISC1
• Gene ID (NCBI): 27185
• RRID: AB_2230451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically.

Publications

Species	Application	Title
mouse	WB	Front Cell Neurosci; Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.; Authors - Anna Sancho-Balsells
mouse	WB	Neurobiol Dis; Hippocampal Pyk2 regulates specific social skills: Implications for schizophrenia; Authors - Laura López-Molina