|Positive WB detected in||A431 cells, HeLa cells, Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
20639-1-AP targets CHCHD6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHCHD6 fusion protein Ag14700|
|Full Name||coiled-coil-helix-coiled-coil-helix domain containing 6|
|Calculated molecular weight||235 aa, 26 kDa|
|Observed molecular weight||26-29 kDa|
|GenBank accession number||BC006123|
|Gene ID (NCBI)||84303|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for CHCHD6 antibody 20639-1-AP||Download protocol|
|IHC protocol for CHCHD6 antibody 20639-1-AP||Download protocol|
|IF protocol for CHCHD6 antibody 20639-1-AP||Download protocol|
|IP protocol for CHCHD6 antibody 20639-1-AP||Download protocol|
|Click here to view our Standard Protocols|
QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.
Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.
QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.
Cell Death Differ
Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
Biochim Biophys Acta Biomembr
Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SITING (Verified Customer) (07-13-2020)
very clear band in our wester blot expose result
Jingwen (Verified Customer) (01-28-2020)
This antibody worked very well when I performed western blot.