IGFBP5 Polyklonaler Antikörper

IGFBP5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 55205-1-AP

Synonyme

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

---

Galerie der Validierungsdaten

• 

  WB analysis of SKOV-3 using 55205-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 55205-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IGFBP5 antibody (55205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, Mausembryogewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

55205-1-AP bindet in WB, IHC, IF, ELISA IGFBP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin-like growth factor binding protein 5
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_000599
• Gene symbol: IGFBP5
• Gene ID (NCBI): 3488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Publikationen

Species	Application	Title
human	IF	Theranostics; Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.; Authors - Sharmy Saimon Mano
human	WB	Oncogene; Fibulin-3 promotes muscle-invasive bladder cancer.; Authors - A L Han
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	WB	Transl Res; PMID: 30392876; Authors - Lin Xihua
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen
rat	WB	Am J Physiol Heart Circ Physiol; Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.; Authors - Guangrui Lai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: CWR22Rv1 cells

IGFBP5 Antibody Immunofluorescence validation (1:100 dilution) in CWR22Rv1 cells (Cat no:55205-1-AP)

Verwandte Produkte

IGF1A-Specific Polyclonal antibody

20214-1-AP

IGFBP2 Polyclonal antibody

11065-3-AP

IGF2BP3 Polyclonal antibody

14642-1-AP

IGFBP4 Polyclonal antibody

18500-1-AP

PAI-1 Polyclonal antibody

13801-1-AP

Vitronectin Polyclonal antibody

15833-1-AP

Thrombospondin 1 Polyclonal antibody

18304-1-AP