• Featured Product
  • KD/KO Validated

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11065-3-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

BP 2, BP2, IBP 2, IBP2, IBP-2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11065-3-AP targets IGFBP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1535

Product name: Recombinant human IGFBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC009902

Sequence: MLPRVGCPALPLPPPPLLPLLPLLLLLLGASGGGGGARAEVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

Predict reactive species
Full Name IGF binding protein 2
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberBC009902
Gene Symbol IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2123101
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).

Protocols

Product Specific Protocols
IHC protocol for IGFBP2 antibody 11065-3-APDownload protocol
IP protocol for IGFBP2 antibody 11065-3-APDownload protocol
WB protocol for IGFBP2 antibody 11065-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Circ Res

Endothelial FUNDC1 Deficiency Drives Pulmonary Hypertension

Authors - Yandong Pei
humanWB

J Clin Invest

A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.

Authors - Xiaochao Tan
  • KD Validated
mouseWB

Nat Commun

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Authors - Hyunho Kim
chickenWB

Oxid Med Cell Longev

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.

Authors - Yafan Gong
mouseWB,IF

Acta Physiol (Oxf)

SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice

Authors - Jingjing Zhang
humanWB,IHC

Front Immunol

The cuproptosis-related signature associated with the tumor environment and prognosis of patients with glioma

Authors - Weichen Wang
Loading...
||
New chat Able
正在加载,请稍候...