IGFBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGFBP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 11065-3-AP

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 11065-3-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11065-3-AP (IGFBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 11065-3-AP

  IP result of anti-IGFBP2 (IP:11065-3-AP, 4ug; Detection:11065-3-AP 1:300) with SH-SY5Y cells lysate 1200ug.

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  IHC staining of human pancreas cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

11065-3-AP targets IGFBP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP2 fusion protein Ag1535
• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC009902
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_2123101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INS-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degraded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers.

Publications

Species	Application	Title
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan ; KD Validated
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim
chicken	WB	Oxid Med Cell Longev; IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.; Authors - Yafan Gong
mouse	WB,IF	Acta Physiol (Oxf); SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice; Authors - Jingjing Zhang
human	IHC,WB	Front Immunol; The cuproptosis-related signature associated with the tumor environment and prognosis of patients with glioma; Authors - Weichen Wang
chicken	WB	Cell Signal; PMID: 28734787; Authors - Jie Yang