IGF1A-Specific Polyclonal antibody

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  IHC staining of mouse liver using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IGF1A-Specific Polyclonal Antibody for IHC, ELISA

Cat No. 20214-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

IGF-1, IGF1, Insulin like growth factor I, IGF-I, IGFI

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Tested Applications

• Positive IHC detected in: mouse liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

20214-1-AP targets IGF1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin-like growth factor 1 (somatomedin C)
• Calculated Molecular Weight: 17 kDa
• Gene Symbol: IGF1
• Gene ID (NCBI): 3479
• RRID: AB_10666736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05019
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.

Publications

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB	J Hepatol; Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: Role of autophagy.; Authors - Xu Xihui X
mouse	WB,IF	Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
mouse	WB,IHC	Int J Cancer; Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.; Authors - Tang Hexiao H
mouse	WB	Mol Neurobiol; Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1(G93A) ALS Mouse Model via Upregulation of D-Amino Acid Oxidase.; Authors - HuiQian Lin
human	WB	Cancer Lett; IFN-γ-mediated IRF1/miR-29b feedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1.; Authors - Li Yuan