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IGF1A-Specific Polyclonal antibody

IGF1A-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20214-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF-1, IGF1A, IGF1A-Specific, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20214-1-AP targets IGF1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor 1 (somatomedin C)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
Gene symbol IGF1
Gene ID (NCBI) 3479
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.

Protocols

Product Specific Protocols
WB protocol for IGF1A-Specific antibody 20214-1-APDownload protocol
IHC protocol for IGF1A-Specific antibody 20214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Authors - Haojie Jin
mouseWB

J Hepatol

Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: Role of autophagy.

Authors - Xu Xihui X
mouseWB,IF

Proc Natl Acad Sci U S A

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Authors - Liang Chen
mouseWB,IHC

Int J Cancer

Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.

Authors - Tang Hexiao H
mouseWB

Mol Neurobiol

Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1(G93A) ALS Mouse Model via Upregulation of D-Amino Acid Oxidase.

Authors - HuiQian Lin
humanWB

Cancer Lett

IFN-γ-mediated IRF1/miR-29b feedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1.

Authors - Li Yuan