IGF2BP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGF2BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

114

Reviews (2)

Cat no : 14642-1-AP

Synonyms

IMP-3, IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3

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Validation Data Gallery

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  WB analysis using 14642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14642-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14642-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 14642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14642-1-AP

  IP result of anti-IGF2BP3 (IP:14642-1-AP, 5ug; Detection:14642-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14642-1-AP

  Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse placenta tissue, BxPC-3 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 39 publications below
• WB: See 92 publications below
• IHC: See 29 publications below
• IF: See 15 publications below
• IP: See 9 publications below
• CoIP: See 2 publications below
• RIP: See 22 publications below

Product Information

14642-1-AP targets IGF2BP3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF2BP3 fusion protein Ag6226
• Full Name: insulin-like growth factor 2 mRNA binding protein 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC065269
• Gene Symbol: IGF2BP3
• Gene ID (NCBI): 10643
• RRID: AB_2122782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Publications

Species	Application	Title
human	WB	Cell; SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.; Authors - Sita Kugel
mouse	WB	Cancer Cell; Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.; Authors - Liem H Nguyen ; KD Validated
mouse	WB	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	RIP	Nat Cell Biol; N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.; Authors - You Wu
human	WB	Mol Cell; m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3; Authors - Ting Shan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

honglei (Verified Customer) (01-17-2023)

This antibody can be used for immunofluorescence and mainly localizes in the nucleus

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: cell line

FH

Jessica (Verified Customer) (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: Neuroblastoma cells (human)

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