HSP60 Polyklonaler Antikörper

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• KD/KO Validated

HSP60 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

111

Kat-Nr. : 15282-1-AP

Synonyme

HSP 60, HSP60, HSP65, HSPD1

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Galerie der Validierungsdaten

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  WB analysis using 15282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15282-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15282-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15282-1-AP

  IP result of anti-HSP60(IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:10000) with HEK-293 cells lysate 1800 ug.

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15282-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (15282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

• 

  FC experiment of HepG2 using 15282-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP60 (15282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 91 publications below
• IHC: See 6 publications below
• IF: See 23 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15282-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA HSP60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Fisch, Hausschwein, Hefe, Huhn, Maus, Ratte, Reis, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSP60 fusion protein Ag7401
• Vollständiger Name: heat shock 60kDa protein 1 (chaperonin)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003030
• Gene symbol: HSPD1
• Gene ID (NCBI): 3329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publikationen

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
mouse	WB	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
mouse	WB	J Hazard Mater; Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy; Authors - Jun Wu
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
mouse	WB,IF	Nat Commun; Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.; Authors - Gustavo Della-Flora Nunes
human	WB	Nat Commun; EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming.; Authors - Fanyan Meng

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