HSP60 Monoclonal antibody

HSP60 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F10E7

Cat no : 66041-1-Ig

Synonyms

Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain, rabbit brain, chicken brain
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human spleen tissue, human tonsillitis tissue, human skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66041-1-Ig is HSP60 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7848
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
WB protocol for HSP60 antibody 66041-1-IgDownload protocol
IHC protocol for HSP60 antibody 66041-1-IgDownload protocol
IF protocol for HSP60 antibody 66041-1-IgDownload protocol
IP protocol for HSP60 antibody 66041-1-IgDownload protocol
FC protocol for HSP60 antibody 66041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Med

The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Authors - Wenzhang Wang
humanWB, IF

Nat Immunol

Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Authors - Jing Geng
humanWB

Autophagy

Substitution of PINK1 Gly411 modulates substrate receptivity and turnover

Authors - Fabienne C Fiesel
mouseWB

J Clin Invest

ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation.

Authors - Mengjie Hu
mouseWB

Autophagy

Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.

Authors - Yinjuan Song
mouseIF

Autophagy

Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage.

Authors - Yuhua Zhang