HERP Polyklonaler Antikörper

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• KD/KO Validated

HERP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10813-1-AP

Synonyme

HERP, HERPUD1, KIAA0025, Mif1, SUP

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Galerie der Validierungsdaten

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  WB analysis using 10813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10813-1-AP (HERP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10813-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 10813-1-AP (HERP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10813-1-AP (HERP antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, LNCaP-Zellen, mit Tunicamycin behandelte HepG2-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10813-1-AP bindet in WB, IF, IHC, ELISA HERP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HERP fusion protein Ag1084
• Vollständiger Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC008320
• Gene symbol: HERPUD1
• Gene ID (NCBI): 9709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

Publikationen

Species	Application	Title
human	WB	Oncogene; Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.; Authors - Chengdong Liu
	WB	J Alzheimers Dis; The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia.; Authors - Zhuo Jia-Min JM
	IF	Biol Reprod; The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome.; Authors - Hao Lanping L
	IHC	PLoS One; C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models.; Authors - Meir Ofir O
	WB	Exp Cell Res; ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.; Authors - Yiman Li ; KD Validated
human	WB	Mol Cell Biochem; Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling.; Authors - Tuusa Jussi T JT

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