CD42b Polyklonaler Antikörper

CD42b Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 12860-1-AP

Synonyme

BSS, CD42b, CD42b alpha, GP1B, GP1BA

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Galerie der Validierungsdaten

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  WB analysis of human peripheral blood platelets using 12860-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig peripheral blood leukocyte using 12860-1-AP

  pig peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 12860-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human peripheral blood platelets, pig peripheral blood leukocyte, human blood
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 1 publications below

Produktinformation

12860-1-AP bindet in WB, IF, IHC, ELISA CD42b und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD42b fusion protein Ag3893
• Vollständiger Name: glycoprotein Ib (platelet), alpha polypeptide
• Berechnetes Molekulargewicht: 626 aa, 69 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC027955
• Gene symbol: CD42b
• Gene ID (NCBI): 2811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Publikationen

Species	Application	Title
human	WB	Adv Healthc Mater; Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury; Authors - Jizong Jiang
rat	WB	Theranostics; A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.; Authors - Peipei Jin
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
mouse	WB	ACS Biomater Sci Eng; Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy.; Authors - Gaoqian Zhao
mouse	IHC	Biochem Pharmacol; GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI.; Authors - Guanxing Pan
mouse	WB	Drug Deliv; Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery.; Authors - Qi-Rui Li

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