EphA4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EphA4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 21875-1-AP

Synonyme

EK8, EPH like kinase 8, EPH receptor A4, EPHA4, Ephrin type A receptor 4, HEK8, SEK, TYRO1, Tyrosine protein kinase TYRO1

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Galerie der Validierungsdaten

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  WB analysis using 21875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21875-1-AP

  IP Result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 21875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuron cells using 21875-1-AP

  IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou.

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  FC experiment of SH-SY5Y using 21875-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug EphA4 antibody (21875-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Neuronzellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 2 publications below

Produktinformation

21875-1-AP bindet in WB, IP, IHC, IF, FC, Cell treatment, ELISA EphA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EphA4 fusion protein Ag16233
• Vollständiger Name: EPH receptor A4
• Berechnetes Molekulargewicht: 986 aa, 110 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC026327
• Gene symbol: EPHA4
• Gene ID (NCBI): 2043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment.; Authors - Shilpa Bhatia
mouse	IHC	Proc Natl Acad Sci U S A; Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity; Authors - Alison Cash
human	WB,IHC	Biochem Biophys Res Commun; Single-chain fragment antibody disrupting the EphA4 function as a therapeutic drug for gastric cancer; Authors - Chen-Wei Chiang
rat	WB	Brain Res Bull; Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats; Authors - Tao Tong
human	IF	bioRxiv; Identification of new ciliary signaling pathways in the brain and insights into neurological disorders; Authors - Abdelhalim Loukil
mouse	FC	JCI Insight; Monocyte pro-inflammatory phenotypic control by Ephrin type-A receptor 4 mediates neural tissue damage.; Authors - Elizabeth A Kowalski

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