• Featured Product
  • KD/KO Validated

Ephrin A3 Polyclonal antibody

Ephrin A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12480-1-AP

Synonyms

EFL 2, EFL2, EFNA3, Ehk1 L, EHK1 ligand, ephrin A3, EPLG3, LERK 3, LERK3



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, A549 cells, rat brain tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12480-1-AP targets Ephrin A3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ephrin A3 fusion protein Ag3188
Full Name ephrin-A3
Calculated Molecular Weight 238 aa, 26 kDa
Observed Molecular Weight 26-35 kDa
GenBank Accession NumberBC017722
Gene Symbol Ephrin A3
Gene ID (NCBI) 1944
RRIDAB_10638305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Ephrin A3 antibody 12480-1-APDownload protocol
IHC protocol for Ephrin A3 antibody 12480-1-APDownload protocol
IP protocol for Ephrin A3 antibody 12480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Research (Wash D C)

Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury

Authors - Yihua Bei
humanWB

Cancer Med

EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma.

Authors - Ruzetuoheti Yiminniyaze
humanWB

Biomed Res Int

OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.

Authors - Hui Wang
  • KD Validated
humanWB,IF

J Exp Clin Cancer Res

The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells

Authors - Marianna Talia
  • KD Validated
ratWB

Brain Res Bull

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats

Authors - Tao Tong
humanWB

J Cell Mol Med

microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3

Authors - Chao Jiang