NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13271-1-AP

Synonyms

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

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Validation Data Gallery

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  WB analysis using 13271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13271-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13271-1-AP

  IP result of anti-NGEF (IP:13271-1-AP, 4ug; Detection:13271-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 13271-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13271-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13271-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13271-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13271-1-AP targets NGEF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGEF fusion protein Ag3967
• Full Name: neuronal guanine nucleotide exchange factor
• Calculated Molecular Weight: 710 aa, 82 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC031573
• Gene Symbol: NGEF
• Gene ID (NCBI): 25791
• RRID: AB_2152381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Brain Res Bull; Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats; Authors - Tao Tong