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  • KD/KO Validated

EDIL3 Polyklonaler Antikörper

EDIL3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12580-1-AP

Synonyme

Integrin-binding protein DEL1, EGF-like repeat and discoidin I-like domain-containing protein 3, Developmentally-regulated endothelial cell locus 1 protein, DEL1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lungengewebe, K-562-Zellen, Maushirngewebe, Mauslungengewebe
Erfolgreiche Detektion in IHChumanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12580-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA EDIL3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EDIL3 fusion protein Ag3274
Vollständiger Name EGF-like repeats and discoidin I-like domains 3
Berechnetes Molekulargewicht 52 kDa
Beobachtetes Molekulargewicht 52-55 kDa
GenBank-ZugangsnummerBC030828
Gene symbol EDIL3
Gene ID (NCBI) 10085
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGF-like repeats and discoidin I-like domain-containing protein 3 (EDIL3), also named as DEL1 or integrin-biding protein DEL1, is a 52-kDa extracellular matrix protein produced by endothelial cells in embryos (PMID: 9420328). It is composed of three EGF repeats and two discoidin I-like domains. The second EGF repeat contains an RGD motif. EDIL3 promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. It may be involved in vascular remodeling during angiogenesis (PMID: 12840057; 14981004). EDIL3 has also been reported to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (PMID: 19008446).

Protokolle

Produktspezifische Protokolle
WB protocol for EDIL3 antibody 12580-1-APProtokoll herunterladen
IHC protocol for EDIL3 antibody 12580-1-APProtokoll herunterladen
IF protocol for EDIL3 antibody 12580-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

J Clin Invest

Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche.

Authors - Ioannis Mitroulis
  • KO Validated
humanELISA

Nat Commun

Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway.

Authors - Tomoki Maekawa
humanWB,IHC

Oncotarget

Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer.

Authors - Shu-Heng Jiang
  • KD Validated

Thromb Haemost

Developmental endothelial locus-1 attenuates complement-dependent phagocytosis through inhibition of Mac-1-integrin.

Authors - Ioannis Mitroulis

Sci Rep

Inhibition of CDK9 as a therapeutic strategy for inflammatory arthritis.

Authors - Annelie Hellvard
humanWB, IHC

Mol Cancer

Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation.

Authors - Ming-Xuan Feng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Guo-rong (Verified Customer) (08-30-2022)

It gives a clear and specific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ARPE-19
EDIL3 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:12580-1-AP)