Dermatopontin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Dermatopontin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 10537-1-AP

Synonyme

Dermatopontin, DPT, TRAMP

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skin using 10537-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10537-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10537-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, humanes Skelettmuskelgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Herzgewebe, humanes Hodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

10537-1-AP bindet in WB, IF, IHC, ELISA Dermatopontin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dermatopontin fusion protein Ag0801
• Vollständiger Name: dermatopontin
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC033736
• Gene symbol: DPT/TRAMP
• Gene ID (NCBI): 1805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the growth inhibition activity of TGF-beta1.

Publikationen

Species	Application	Title
human	IHC	Oncotarget; DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling.; Authors - Ying Fu
human	WB,IHC	Int J Cancer; Dermatopontin: a potential predictor for metastasis of human oral cancer.; Authors - Yamatoji Masanobu M
mouse	IF	Am J Physiol Cell Physiol; Single-cell Transcriptomic Analyses Identifies Extensive Heterogeneity in the Cellular Composition of Mouse Achilles Tendons.; Authors - Andrea J De Micheli
human	IHC	BMC Cancer; QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study.; Authors - Hinsch Nora N
human	WB	Int J Oncol; NRG1 regulates redox homeostasis via NRF2 in papillary thyroid cancer.; Authors - Ting-Ting Zhang ; KD Validated
human	WB,IHC	Ann Transl Med; Identification of the distinctive role of DPT in dilated cardiomyopathy: a study based on bulk and single-cell transcriptomic analysis.; Authors - Yang Lu

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