Collagen Type I Polyklonaler Antikörper
Collagen Type I Polyklonal Antikörper für FC, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human und mehr (6)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14695-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Hausschwein-Hautgewebe, humanes Plazenta-Gewebe |
Erfolgreiche Detektion in IHC | humanes Hautkrebsgewebe, humanes Kolongewebe, humanes Ösophaguskarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in FC | SW480-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 617 publications below |
IHC | See 157 publications below |
IF | See 115 publications below |
IP | See 1 publications below |
FC | See 2 publications below |
Produktinformation
14695-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Collagen Type I und zeigt Reaktivität mit Hausschwein, human
Getestete Reaktivität | Hausschwein, human |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Collagen Type I fusion protein Ag6281 |
Vollständiger Name | collagen, type I, alpha 2 |
Berechnetes Molekulargewicht | 1366 aa, 130 kDa |
Beobachtetes Molekulargewicht | 120-130 kDa |
GenBank-Zugangsnummer | BC054498 |
Gene symbol | COL1A2 |
Gene ID (NCBI) | 1278 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.
Protokolle
Produktspezifische Protokolle | |
---|---|
WB protocol for Collagen Type I antibody 14695-1-AP | Protokoll herunterladen |
IHC protocol for Collagen Type I antibody 14695-1-AP | Protokoll herunterladen |
FC protocol for Collagen Type I antibody 14695-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
ACS Nano Penetration Cascade of Size Switchable Nanosystem in Desmoplastic Stroma for Improved Pancreatic Cancer Therapy. | ||
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Nat Protoc Responsive and activable nanomedicines for remodeling the tumor microenvironment. | ||
J Hazard Mater Crystalline silica-induced macrophage pyroptosis interacting with mitophagy contributes to pulmonary fibrosis via modulating mitochondria homeostasis | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Brice-Emmanuel (Verified Customer) (10-12-2023) | I have 2 bands in 70 kDa
|
FH S (Verified Customer) (03-20-2023) | Excellent Antibody!
|
FH Balawant Kumar (Verified Customer) (12-20-2022) | antibody is working great. I have used this antibody for mice colon tissue and colon cancer cell line
|
FH PK (Verified Customer) (07-08-2022) | Very Good
|
FH Ren Jie (Verified Customer) (06-22-2022) | Very strong signal for collagen I, needs stringent wash conditions to minimize ladder staining in western blot
|
FH S (Verified Customer) (12-01-2021) | Excellent
|
FH Hala (Verified Customer) (02-23-2021) | very good
|
FH Eistine (Verified Customer) (05-31-2019) | The antibody works pretty good
|