Collagen Type XVI Polyclonal antibody

Collagen Type XVI Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19802-1-AP

Synonyms

COL16A1, COL16, FP1572, Collagen alpha-1(XVI) chain, Collagen alpha 1(XVI) chain

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Validation Data Gallery

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  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skin using 19802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Collagen Type XVI antibody (19802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IF-P detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19802-1-AP targets Collagen Type XVI in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen, type XVI, alpha 1
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 158 kDa
• GenBank Accession Number: NM_001856
• Gene Symbol: COL16A1
• Gene ID (NCBI): 1307
• RRID: AB_10638606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL16A1, also named as FP1572, belongs to the fibril-associated collagens with interrupted helices (FACIT) family. COL16A1 is involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology. The antibody is specific to COL16A1.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); MicroRNA-152 and -181a participate in human dermal fibroblasts senescence acting on cell adhesion and remodeling of the extra-cellular matrix.; Authors - Mancini Mara M