CDK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

272

Rezensionen (1)

Kat-Nr. : 10122-1-AP

Synonyme

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

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Galerie der Validierungsdaten

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  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10122-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10122-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10122-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10122-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10122-1-AP

  IP result of anti-CDK2 (IP:10122-1-AP, 4ug; Detection:10122-1-AP 1:10000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 10122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (10122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 10122-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (10122-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 265 publications below
• IHC: See 14 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below

Produktinformation

10122-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA CDK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK2 fusion protein Ag0166
• Vollständiger Name: cyclin-dependent kinase 2
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC003065
• Gene symbol: CDK2
• Gene ID (NCBI): 1017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,CoIP	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo ; KD Validated
human	WB,IHC	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Silvia (Verified Customer) (10-19-2021)

It works well for WB

• Applications: Western Blot
• Cell Tissue Type: Human fibroblasts

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