cIAP1 Polyklonaler Antikörper

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• KD/KO Validated

cIAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 10022-1-AP

Synonyme

API1, BIRC2, MIHB, RNF48, C-IAP1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skeletal muscle using 10022-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10022-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10022-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10022-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10022-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10022-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10022-1-AP (cIAP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10022-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using cIAP1 antibody (10022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10022-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug cIAP1 Polyclonal antibody (10022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Jurkat-Zellen, Maushirngewebe, Maushodengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Pankreasgewebe, Maushodengewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10022-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA cIAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cIAP1 fusion protein Ag15203
• Vollständiger Name: baculoviral IAP repeat-containing 2
• Berechnetes Molekulargewicht: 618 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 55-60 kDa
• GenBank-Zugangsnummer: BC016174
• Gene symbol: cIAP1
• Gene ID (NCBI): 329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.

Publikationen

Species	Application	Title
zebrafish	WB,IF	Nat Genet; Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.; Authors - Santoro Massimo M MM ; KD Validated
human	IF	Mol Cell; Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.; Authors - Mingjian Du
human	WB	Mol Cell; A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.; Authors - Michael Hinz ; KD Validated
mouse	WB	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
human	WB	Dev Cell; Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.; Authors - Maria Zachari
mouse	WB	J Immunol; Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.; Authors - Peters Anna L AL

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