cIAP2 Polyclonal antibody

cIAP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 24304-1-AP

Synonyms

AIP1, API2, Apoptosis inhibitor 2, BIRC3, C IAP2, CIAP2, HAIP1, hIAP 1, HIAP1, IAP 1, IAP homolog C, IAP1, MALT2, MIHC, RING finger protein 49, RNF49

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Validation Data Gallery

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  WB analysis using 24304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 24304-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 24304-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Daudi using 24304-1-AP

  IP result of anti-cIAP2 (IP:24304-1-AP, 4ug; Detection:24304-1-AP 1:1000) with Daudi cells lysate 2800ug.

• 

  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Daudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells
• Positive IP detected in: Daudi cells
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 5 publications below

Product Information

24304-1-AP targets cIAP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: cIAP2 fusion protein Ag20143
• Full Name: baculoviral IAP repeat-containing 3
• Calculated Molecular Weight: 604 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC027485
• Gene Symbol: cIAP2
• Gene ID (NCBI): 330
• RRID: AB_2879485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Dev Cell; Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.; Authors - Maria Zachari
human	WB	Oncotarget; The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.; Authors - Tian-Hao Liu
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human,mouse	WB	Biomed Pharmacother; Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.; Authors - Lei Wang
human	IHC	Cancer Med; Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.; Authors - Shu-Mei Chen
human	WB	Cancer Biol Ther; Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.; Authors - Liu Qin Q