Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.
IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.
Western Blot (WB) analysis of His-eGFP-V5 added to HEK293T cell extract
The anti-V5 antibody [SV5-P-K] is a recombinant, monoclonal mouse IgG1, has very low background and detects even low amounts of V5-tagged proteins. Secondary antibody ChromoTek’s Alpaca anti-mouse IgG1 Alexa Fluor 488 Nano-Secondary.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.