ChromoTek V5-Trap® Magnetic Agarose Kit

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Publications

1

Cat no : v5tmak

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

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Validation Data Gallery

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  V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

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  Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

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  V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

• 

  Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

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Product Information

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

• Description: The V5-Trap Magnetic Agarose Kit contains V5-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP, Protein purification
• Specificity/Target: V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
• Binding capacity: 17.5 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
• Magnetic agarose: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: V5-peptide; SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 40 nM
• Compatibility with mass spectrometry: The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2868498
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component	Description
V5-Trap™ Magnetic Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate

Other Formats

V5-Trap Agarose Kit

V5-Trap Agarose

V5-Trap Magnetic Agarose

V5-Trap Magnetic Particles M-270 Kit

V5-Trap Magnetic Particles M-270

Publications

Application	Title
ChIP	Cell Rep; PAPAS promotes differentiation of mammary epithelial cells and suppresses breast carcinogenesis; Authors - Sijia Ren