V5-tag Recombinant antibody

V5-tag Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240529D8

Cat no : 81775-4-RR

Synonyms

240529D8

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Validation Data Gallery

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  WB analysis using 81775-4-RR

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 81775-4-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 81775-4-RR (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells, Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81775-4-RR targets V5-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: V5-tag
• Calculated Molecular Weight: 1 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.