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MMP13 Recombinant antibody, PBS Only (Detector)

MMP13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240060B10

Cat no : 83188-2-PBS

Synonyms

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00266-1

    Sandwich ELISA standard curve of MP00266-1

    Sandwich ELISA standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. 83188-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag34892. 83188-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MMP13 Recombinant antibody (83188-2-RR)

    IF Staining of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (83188-2-RR, Clone: 240060B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using MMP13 Recombinant antibody (83188-2-RR)

    FC experiment of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-2-RR, Clone:240060B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00266-1

    Cytometric bead array standard curve of MP00266-1

    Cytometric bead array standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. PBS Only. Capture antibody: 83188-3-PBS. Detection antibody: 83188-2-PBS. Standard: Ag34892. Range: 5-40 ng/mL.

  • Cytometric bead array standard curve of MP00266-2

    Cytometric bead array standard curve of MP00266-2

    Cytometric bead array standard curve of MP00266-2, MMP13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83188-1-PBS. Detection antibody: 83188-2-PBS. Standard: Ag34892. Range: 5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83188-2-RR

    Affinity and Kinetic Characterization of 83188-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-2-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

Product Information

83188-2-PBS targets MMP13 as part of a matched antibody pair:

MP00266-1: 83188-3-PBS capture and 83188-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00266-2: 83188-1-PBS capture and 83188-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MMP13 fusion protein Ag34892
Full Name matrix metallopeptidase 13
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight 70 kDa, 54 kDa
GenBank Accession NumberBC074808
Gene Symbol MMP13
Gene ID (NCBI) 4322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).