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MMP13 Recombinant antibody

MMP13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240060B10

Cat no : 83188-2-RR

Synonyms

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MMP13 Recombinant antibody (83188-2-RR)

    IF Staining of MCF-7 using 83188-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (83188-2-RR, Clone: 240060B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using MMP13 Recombinant antibody (83188-2-RR)

    FC experiment of MCF-7 using 83188-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-2-RR, Clone:240060B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83188-2-RR

    Affinity and Kinetic Characterization of 83188-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-2-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83188-2-RR targets MMP13 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MMP13 fusion protein Ag34892
Full Name matrix metallopeptidase 13
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight 70 kDa, 54 kDa
GenBank Accession NumberBC074808
Gene Symbol MMP13
Gene ID (NCBI) 4322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).

Protocols

Product Specific Protocols
IF protocol for MMP13 antibody 83188-2-RRDownload protocol
FC protocol for MMP13 antibody 83188-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols