CoraLite® Plus 647 Anti-Human uPAR/CD87 Rabbit Recombinant Antibody

uPAR/CD87 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240366G10

Cat no : CL647-98048

Synonyms

PLAUR, UPAR, U PAR, MO3, CD87

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Validation Data Gallery

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  FC experiment of U-937 using CL647-98048

  1x10^6 PMA treated U-937 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (CL647-98048, Clone:240366G10) (red), or Isotype Control (blue). 1x10^6 untreated U-937 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (CL647-98048, Clone:240366G10) (black, dashed). Cells were not fixed.

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Tested Applications

• Positive FC detected in: PMA treated U-937 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98048 is uPAR/CD87 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_002659.4
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)