Anti-Human uPAR/CD87 Rabbit Recombinant Antibody

uPAR/CD87 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240366G10

Cat no : 98048-1-RR

Synonyms

PLAUR, UPAR, U PAR, MO3, CD87

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Validation Data Gallery

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  FC experiment of U-937 using 98048-1-RR

  1x10^6 untreated (black) or PMA-treated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) and PE-conjugated Goat Anti-Rabbit IgG (red). 1x10^6 PMA treated U-937 cells were surface stained with 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98048-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98048-1-RR against Human uPAR were performed. The affinity constant is 4.70 nM.

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Tested Applications

• Positive FC detected in: PMA treated U-937 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98048-1-RR is uPAR/CD87 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_002659.4
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)