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Biotin-conjugated t-Plasminogen activator/tPA Polyclonal antibody
t-Plasminogen activator/tPA Polyclonal Antibody for IHC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC
Conjugate
Biotin
Cat no : Biotin-10147
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-10147 targets t-Plasminogen activator/tPA in IHC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | t-Plasminogen activator/tPA fusion protein Ag0200 |
Full Name | plasminogen activator, tissue |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 32-35 kDa, 65 kDa |
GenBank Accession Number | BC002795 |
Gene Symbol | tPA |
Gene ID (NCBI) | 5327 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin t-Plasminogen activator/tPA antibody Biotin-10147 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |