tPA Polyclonal antibody

• Featured Product
• KD/KO Validated

tPA Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 10147-1-AP

Synonyms

Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA

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Validation Data Gallery

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  WB analysis using 10147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10147-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10147-1-AP

  IP Result of anti-tPA (IP:10147-1-AP, 3ug; Detection:10147-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10147-1-AP (tPA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10147-1-AP (tPA antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse brain using 10147-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10147-1-AP (tPA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse pancreas tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Product Information

10147-1-AP targets tPA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: tPA fusion protein Ag0200
• Full Name: plasminogen activator, tissue
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 32-35 kDa, 65 kDa
• GenBank accession number: BC002795
• Gene symbol: PLAT
• Gene ID (NCBI): 5327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Redox Biol; FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.; Authors - Ying Cai ; KO Validated
mouse	IF	J Neurosci; Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice.; Authors - V Swaroop Achuta
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma
mouse	IHC	Glia; Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.; Authors - Ewa E Bres
human	WB	Sci Rep; Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.; Authors - Yang Liu
mouse	IF	Cell Cycle; tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.; Authors - Ling Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

nuo (Verified Customer) (08-22-2021)

There is a band at around 64 kD as expected, although there were other nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

tPA Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10147-1-AP)