• Featured Product
  • KD/KO Validated

tPA Polyclonal antibody

tPA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10147-1-AP

Synonyms

Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA, t-Plasminogen activator



Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10147-1-AP targets tPA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen tPA fusion protein Ag0200
Full Name plasminogen activator, tissue
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 32-35 kDa, 65 kDa
GenBank Accession NumberBC002795
Gene Symbol PLAT
Gene ID (NCBI) 5327
RRIDAB_2299701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Protocols

Product Specific Protocols
WB protocol for tPA antibody 10147-1-APDownload protocol
IHC protocol for tPA antibody 10147-1-APDownload protocol
IF protocol for tPA antibody 10147-1-APDownload protocol
IP protocol for tPA antibody 10147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Redox Biol

FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.

Authors - Ying Cai
  • KO Validated
mouseIF

J Neurosci

Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice.

Authors - V Swaroop Achuta
humanWB

Aging (Albany NY)

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Authors - Pingping Ma
mouseIHC

Glia

Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.

Authors - Ewa E Bres
humanWB

Sci Rep

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Authors - Yang Liu
mouseIF

Cell Cycle

tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.

Authors - Ling Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

nuo (Verified Customer) (08-22-2021)

There is a band at around 64 kD as expected, although there were other nonspecific bindings.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate
tPA Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10147-1-AP)