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  • KD/KO Validated

tPA Polyclonal antibody

tPA Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10147-1-AP


Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA

Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10147-1-AP targets tPA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen tPA fusion protein Ag0200
Full Name plasminogen activator, tissue
Calculated molecular weight 57 kDa
Observed molecular weight 32-35 kDa, 65 kDa
GenBank accession numberBC002795
Gene symbol PLAT
Gene ID (NCBI) 5327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.


Product Specific Protocols
WB protocol for tPA antibody 10147-1-APDownload protocol
IHC protocol for tPA antibody 10147-1-APDownload protocol
IF protocol for tPA antibody 10147-1-APDownload protocol
IP protocol for tPA antibody 10147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Redox Biol

FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.

Authors - Ying Cai
  • KO Validated

J Neurosci

Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice.

Authors - V Swaroop Achuta

Aging (Albany NY)

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Authors - Pingping Ma


Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.

Authors - Ewa E Bres

Sci Rep

Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.

Authors - Yang Liu

Cell Cycle

tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.

Authors - Ling Chen


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


nuo (Verified Customer) (08-22-2021)

There is a band at around 64 kD as expected, although there were other nonspecific bindings.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate
tPA Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10147-1-AP)