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CoraLite® Plus 750-conjugated spike protein Recombinant antibody

spike protein Recombinant Antibody for

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4I18

Cat no : CL750-82734-4

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated spike protein Recomb (CL750-82734-4)

    WB analysis using CL750-82734-4

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82734-4 (spike protein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Information

CL750-82734-4 targets spike protein in applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen spike protein fusion protein Ag30684
Full Name SARS-CoV-2 Spike Protein
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.