spike protein Recombinant antibody

spike protein Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4I18

Cat no : 82734-4-RR

Synonyms

RBD, COVID-19 RBD, Spike, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike

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Validation Data Gallery

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  WB analysis of Recombinant protein using 82734-4-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82734-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82734-4-RR against Virus spike protein were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82734-4-RR targets spike protein in WB, ELISA applications and shows reactivity with virus samples.

• Tested Reactivity: virus
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: spike protein fusion protein Ag30684
• Full Name: SARS-CoV-2 Spike Protein
• Calculated Molecular Weight: 141 kDa
• GenBank Accession Number: NC_045512
• Gene Symbol: SARS-COV-2
• Gene ID (NCBI): 43740568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.