smooth muscle actin specific Monoclonal antibody, PBS Only

smooth muscle actin specific Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A11

Cat no : 67735-1-PBS

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, smooth muscle actin specific, α-sma

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Validation Data Gallery

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  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig vein using 67735-1-Ig (same clone as 67735-1-PBS)

  pig vein tissue were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IF Staining of rat heart using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67735-1-PBS targets smooth muscle actin specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.